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The significantly acclaimed laboratory usual, Methods in Enzymology, is among the so much hugely revered courses within the box of biochemistry. in view that 1955, every one quantity has been eagerly awaited, usually consulted, and praised via researchers and reviewers alike. The sequence includes a lot fabric nonetheless correct at the present time - really a vital book for researchers in all fields of existence sciences
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Extra resources for Nucleic Acids, Part D
G. Stokes, Phil. Trans. Roy. Soc. London, 142, 463 (1852). 5G. N. Lewis and M. Kasha, J. Amer. Chem. Soc. 66, 2100 (1944). 3knr IA FIe, 1. The Jablonski diagram showing energy levels of the ground state (IA), and lowest excited singlet (IA*) and triplet states (SA*)of an aromatic molecule, along with the typical lifetimes of these states. Nonradiative processes are shown as wavy lines. , are the radiative and nonradiative deexcitation rates, of the excited singlet state, respectively, while 8kr and skn, are the corresponding rates for the triplet state.
Eisinger and G. Navon, J. Chem. Phys. 50, 2069 (1969). 34 TECHNIQUES FOR STRUCTURAL ANALYSIS  84"K (Xl/5) 300 400 350 450 Wavelength(nm) FIG. ------ ' ~_~ / c~ 340 oE3 -~ 320 0 I 4 1 I 5 6 I 7 I I I I I 8 9 I0 II 12 I 13 300 103/7"(OK) Fie. 4 FIGs. 3 a n d 4. Figure 3 shows t h e fluorescence spectra of p r o t o n a t e d adenine in E G W a t various t e m p e r a t u r e s . T h e lower p a r t of Fig. 4 shows t h e fluorescence yield a n d w a v e l e n g t h of m a x i m u m emission as a function of t h e inverse absolute t e m p e r a t u r e .
A good illustration of solvent reorientation is afforded by the temperature and viscosity dependence of the fluorescence of protonated purines. 19-~8Figures 3 and 4 show that at low temperatures both the quan19 j . Eisinger, Photochem. Photobiol. 9, 247 (1969). 20 H. C. B~rresen, Acta Chem. ~cand. 21, 2463 (1967).  LUMINESCENCE SPECTROSCOPY 35 rum yield and the wavelength maximum of the emission spectrum of protonated adenine are constant. At about 125°K where the ethyleneglycolwater glass begins to soften, the fluorescence starts to shift to the red and does not become temperature independent again until 180°K is reached.